WGS of Dirofilaria immitis: single microfilaria from Queensland, Aust... - SRA

SRX24191778: WGS of Dirofilaria immitis: single microfilaria from Queensland, Australia
1 ILLUMINA (Illumina NovaSeq 6000) run: 5.5M spots, 1.6G bases, 580.2Mb downloads

Design: Single microfilariae were isolated from dog blood and whole genome amplification was performed using the GenomePlex Single Cell Whole Genome Amplification Kit (Sigma-Aldrich, Australia). DNA was purified with the QIAquick PCR Purification Kit (QIAGEN, Australia). DNA samples were sent to Novogene for library construction and Illumina whole genome sequencing.

Submitted by: The University of Sydney

Study: Whole genome sequencing of individual Dirofilaria immitis microfilariae

PRJNA1097760 • SRP500409 • All experiments • All runs

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This study involved whole genome amplification and whole-genome sequencing of single Dirofilaria immitis microfilariae samples isolated from dog blood. Accessing these immature life stages of D. immitis is less invasive and more feasible than extracting the adult stages. Sequencing these individual microfilariae provides opportunities for conducting genome-wide populations studies.

Sample: Illumina HiSeq PE150 using DNA extracted from single microfilariae. Microfilariae were collected from dog blood and contain host DNA.

SAMN40887499 • SRS20965753 • All experiments • All runs

Organism: Dirofilaria immitis

Library:

Name: FDSW210306867-1r

Instrument: Illumina NovaSeq 6000

Strategy: WGA

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 5.5M spots, 1.6G bases, 580.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR28592519	5,482,894	1.6G	580.2Mb	2024-04-09

ID:: 32503303

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